Most restriction enzymes bind palindromes.

So both 5'CCGG or 3'GGCC would have been acceptable in this scenario.

Restriction enzymes cut at palindromic sequences... i knew this would bite me in the ass someday.+ We are testing two pieces of DNA- one with a mutation, one without.+ We need to use a restriction enzyme to cut exactly where the mutation is in order to see which piece of DNA has the sequence of interest (the restriction enzyme site). I chose 5' ACCG, which would cut the mutated strand and not the wild type. Why is this wrong? because when you write the complementary strand you get TGGC, which is not a mirror image of ACCG. + Correct answer 5' CCGG, the complementary strand: GGCC. This is a palindromic sequence (1), would cut the mutated strand of DNA and not the wild type (2), which when using gel electrophoresis the mutated strand would show 2 bands small bands, while the non mutated strand would show one large band (3)